Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues
作者： Mariana Bustamante , Jian Jin , Oriol Casagran , Tania Nolan , José Luis Riechmann
DOI: 10.1007/978-1-4614-9408-9_21
关键词:
摘要: Real-time, or quantitative, reverse transcription polymerase chain reaction (qRT-PCR), is a powerful method for rapid and reliable quantification of mRNA abundance. Although it has not featured prominently in flower development research the past, availability novel techniques synchronized induction development, isolation cell-specific populations, suggests that detailed quantitative analyses gene expression over time specific tissues cell types by qRT-PCR will become more widely used. In this chapter, we discuss considerations studying using qRT-PCR, such as identification suitable reference genes experimental setup addition, provide protocols performing experiments multiwell plate format (with LightCycler(®) 480 system, Roche) with nanofluidic arrays (BioMark™ Fluidigm), which allow automatic combination sets samples assays, significantly reduce volume number liquid-handling steps performed during experiment.
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