Recombination and expression of a cloned fragment of the DNA of Bacillus subtilis bacteriophage SP01

摘要: Abstract Fragments of the DNA bacteriophage SPO1 can be cloned in Bacillus subtilis , natural host for SPO1. Clones same fragment are either stable or unstable, depending on vector used. contains hydroxymethyluracil instead thymine. The fragments do not contain hydroxymethyluracil, even when it is present cell as a consequence SPOT infection. Yet active both expression its genetic information and recombination with superinfecting DNA. gene 17, which probably late gene, expressed from regardless orientation insertion, suggesting that transcribed promoter.