Schistosoma mansoni ex vivo lung-stage larvae excretory–secretory antigens as vaccine candidates against schistosomiasis

摘要: Schistosoma mansoni lung-stage larvae are known to be the major target of innate and acquired immunity schistosomiasis. Lung schistosomula cytosolic or surface membrane antigens hidden, entirely inaccessible host immune system, hence not particularly important as vaccine candidates. Conversely, excretory-secretory (E-S) products released from intact, viable, elongated, contractile ideal potential vaccines, such molecules can readily play a central role in induction local primary memory response effectors that would directly target, surround, pursue while negotiating lung capillaries. Therefore, 6-day-old ex vivo were isolated mouse hamster cells used for generation E-S products, which shown elicit strong responses significant (P<0.05) protection against challenge infection BALB/c mice. Proteomic analysis following 10x concentration sodium dodecyl sulfate-polyacrylamide gel electrophoresis identified peptides related innumerable about 15 S. mansoni-specific proteins. Selected prepared multiple antigen peptide (MAP) recombinant form stimulate considerable specific antibody peripheral blood mononuclear cell expression mRNA several cytokines immunized C57BL/6 However, highly (P<0.05 <0.005) reduction worm burden egg load was recorded only when immunization conditions test mice provided antigen-specific T helper (Th) type milieu favorable each immunogen. That polarized Th1 aldolase thioredoxin peroxidase 1 MAPs, Th2 14-3-3-like protein, mixed Th1/Th17 calpain MAP, Th1/Th2 p18 protein. The findings together indicated issue is critical nature source development