miR‐214 activates TP53 but suppresses the expression of RELA, CTNNB1, and STAT3 in human cervical and colorectal cancer cells

摘要: High Mobility Group AT-hook 1 (HMGA1) was identified as a target of miR-214 in human cervical and colorectal cancers (CaCx CRC) previous study. While the expression remains suppressed, HMGA1 behaves potent oncogene plays crucial roles several aberrant signalling pathways by interacting with intermediates like RELA, CTNNB1, STAT3, TP53 CaCx CRC. Hypothetically, should be able to regulate stabilization some these through regulation HMGA1. This assessed ectopically expressing or complementarily, inhibiting In promoter luciferase assays, inhibited NF-κB Wnt activities but elevated activity cancer cells. Further, suppressed HMGA1, STAT3 while elevating levels, similar when small interfering RNA (siRNA) against used, revealed Western blotting. It is suggested that poor miR-214, commonly reported CRC tissues, may not only result sustained also congruent suppression during initiation/progression. These states are, however, reversed reintroduced could explain tumour suppressive functions observed earlier studies. Further studies required reveal how microRNA-mediated affect individual Current results its direct target, few TP53, which interacts. play deregulated Hence, it proposed might act suppressor regulating knowledge has potential help design novel therapeutic strategies