Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue.

摘要: Matrix-assisted laser desorption ionization image mass spectrometry (MALDI-IMS) has been developed for the identification of peptides in various tissues. The MALDI-IMS signal distribution patterns and quantification intensities regions interest (ROI) with healthy were compared disease specific biomarkers. We performed a new ROI analysis using conventional t-test data number independent Cohen’s d-value analysis. Using these techniques, we analysed heart tissues after acute myocardial infarction (AMI). As result, IMS signals mitochondrial adenosine triphosphate synthase alpha subunit (ATP5A), myosin-6/7(MYH6/7), aortic actin, myosin light chain 3 (MYL3) identified infarcted region. In particular, MYH7 are significantly greater region may be promising technique biomarkers pathological studies that involve comparison diseased control areas.