ATP synthase subunit alpha and LV mass in ischaemic human hearts

摘要: Mitochondrial dysfunction plays a critical role in the development of ischaemic cardiomyopathy (ICM). In this study, mitochondrial proteome cardiac tissue ICM patients was analysed by quantitative differential electrophoresis (2D-DIGE) and mass spectrometry (MS) for first time to provide new insights into cardiomyopathy. We isolated mitochondria from LV samples explanted hearts (n = 8) control donors used proteomic approach investigate variations protein expression. found that most altered proteins were involved energy metabolism (82%). focused on ATPA, which is production, dihydrolipoyl dehydrogenase, implicated substrate utilization, observed these molecules overexpressed changes detected processes mediated closely related. Notably, we ATPA overexpression associated with reduction (r = −0.74, P < 0.01). also substantial increase expression elongation factor Tu, molecule synthesis, PRDX3, stress response. All validated using classical techniques novel precise selected reaction monitoring analysis an RNA sequencing approach, total heart being increased 24. This study provides key enhance our understanding cellular mechanisms related pathophysiology could lead aetiology-specific failure therapies. serve as molecular target suitable therapeutic interventions.