miR-21 inhibits the effects of cyclooxygenase-2 inhibitor NS398 on apoptosis and invasion in gastric cancer cells.

摘要: OBJECTIVE To investigate the role of miR-21 in cyclooxygenase-2 inhibitor NS398-induced apoptosis and invasion gastric cancer (GC) cells. METHODS AGS cells were treated with NS398 transfected miR-21. Quantitative real-time polymerase chain reaction was used to measure mRNA expression. Apoptotic assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling flow cytometric analysis. The protein expression cleaved caspase-3, Bcl-2, Bax, Bak, PTEN detected Western blot. capacities for migration measured transwell wound-healing assays, respectively. RESULTS Treatment induced a dose-dependent manner accompanied significant downregulation Upregulation transfection mimics into blocked apoptosis. changes Bcl-2 family members, showing an increase PTEN, concomitant decrease Bcl-2. In mimics, these reversed. cellular invasiveness upregulation CONCLUSION mediates anticancer effects GC regulating apoptosis-related proteins. is one molecular targets this specific prevention treatment GC.