Isolation and characterization of a cartilage-specific membrane antigen (CH65) : comparison with cytokeratins and heat-shock proteins
作者： W. Van Eden , S. H. E. Kaufmann , A. Schulmeister , A. Wand-Württenberger , H. Bang
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摘要: We report the isolation and characterization of a 65,000 MW chondrocyte autoantigen (CH65) which may be involved in rheumatoid arthritis. This chondrocyte-specific antigen reacted with sera from patients arthritis (RA). CH65 did not cross-react polyclonal antibody raised against microbial heat-shock protein (hsp) 65, anti-human hsp 65 monoclonal antibodies (mAb) (LK1 LK2), anti-microbial mAb (IA10, IIC8 WTB-78H1) anti-cytokeratin 8, 18, 19 (NCL5D3MAb). could purified chicken membranes by ammonium sulphate precipitation novel electro-gel-filtration method. The amino acid analysis yielded an unusually high degree glycine, serine asparagine residues. internal sequence obtained tryptic digestion revealed homologies cytokeratin family. Despite these homologies, lacked immunological cross-reactivity commercial antibodies. Mice generated (C6) were used to identify as tissue-specific constitutive membrane chondrocytes. Sera RA cross-reacted CH65. stress or (hsp 65), implicated development experimental clinical arthritis, showed no Western blots. These findings suggest that represent interesting cartilage-specific new RA. availability this form specific offer useful tools research.
参考文章(7)
S. H. E. Kaufmann, B. Schoel, A. Wand-Württenberger, U. Steinhoff, M. E. Munk, T. Koga, T-Cells, Stress Proteins, and Pathogenesis of Mycobacterial Infections T-Cell Paradigms in Parasitic and Bacterial Infections. ,vol. 155, pp. 125- 141 ,(1990) , 10.1007/978-3-642-74983-4_9
C. Baboonian, D. Halliday, P. J. W. Venables, T. Pawlowski, G. Millman, R. N. Maini, Antibodies in rheumatoid arthritis react specifically with the glycine alanine repeat sequence of Epstein-Barr nuclear antigen-1 Rheumatology International. ,vol. 9, pp. 161- 166 ,(1989) , 10.1007/BF00271874
Irun R Cohen, Douglas B Young, Autoimmunity, microbial immunity and the immunological homunculus Immunology Today. ,vol. 12, pp. 105- 110 ,(1991) , 10.1016/0167-5699(91)90093-9
Eng M. Tan, Edward K.L. Chan, Kevin F. Sullivan, Robert L. Rubin, Antinuclear antibodies (ANAs): diagnostically specific immune markers and clues toward the understanding of systemic autoimmunity. Clinical Immunology and Immunopathology. ,vol. 47, pp. 121- 141 ,(1988) , 10.1016/0090-1229(88)90066-9
R. B. CLAGUE, Autoantibodies to cartilage collagens in rheumatoid arthritis. Do they perpetuate the disease or are they irrelevant Rheumatology. ,vol. 28, pp. 1- 4 ,(1989) , 10.1093/RHEUMATOLOGY/28.1.1
G. TSOULFA, G. A. W. ROOK, G. M. BAHR, M. A. SATTAR, K. BEHBEHANI, D. B. YOUNG, A. MEHLERT, J. D. A. VAN-EMBDEN, F. C. HAY, D. A. ISENBERG, P. M. LYDYARD, Elevated IgG antibody levels to the mycobacterial 65-kDa heat shock protein are characteristic of patients with rheumatoid arthritis. Scandinavian Journal of Immunology. ,vol. 30, pp. 519- 527 ,(1989) , 10.1111/J.1365-3083.1989.TB02459.X
M J Fritzler, M Salazar, Diversity and origin of rheumatologic autoantibodies. Clinical Microbiology Reviews. ,vol. 4, pp. 256- 269 ,(1991) , 10.1128/CMR.4.3.256