A Conserved Mechanism for Control of Human and Mouse Embryonic Stem Cell Pluripotency and Differentiation by Shp2 Tyrosine Phosphatase
作者： Dongmei Wu , Yuhong Pang , Yuehai Ke , Jianxiu Yu , Zhao He
DOI: 10.1371/JOURNAL.PONE.0004914
关键词:
摘要: Recent studies have suggested distinctive biological properties and signaling mechanisms between human mouse embryonic stem cells (hESCs mESCs). Herein we report that Shp2, a protein tyrosine phosphatase with two SH2 domains, has conserved role in orchestration of intracellular cascades resulting initiation differentiation both hESCs mESCs. Homozygous deletion Shp2 mESCs inhibited into all three germ layers, siRNA-mediated knockdown expression led to similar phenotype impaired differentiation. A small molecule inhibitor enzyme suppressed hESC mESC capacity. modulates Erk, Stat3 Smad pathways ES and, particular, regulates BMP4-Smad pathway bi-directionally hESCs. These results reveal common mechanism shared by ESCs via modulation overlapping divergent pathways.
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