Cholesteryl butyrate solid lipid nanoparticles inhibit adhesion of human neutrophils to endothelial cells

摘要: 1 Adhesion of polymorphonuclear cells (PMNs) to vascular endothelial (EC) is a critical step in recruitment and infiltration leukocytes into tissues during inflammation. High doses butyric acid have been shown ameliorate inflammation inflammatory bowel diseases (IBD). Cholesteryl-butyrate solid lipid nanoparticles (chol-but SLN) as prodrug are possible delivery system for acid. 2 Sodium butyrate or chol-but SLN were coincubated with human PMNs umbilical vein EC (HUVEC); adhesion was quantified by computerized microimaging fluorescence analysis. Both sodium displayed antiadhesive effects on FMLP- IL-1β-stimulated concentration–response curve (10−8–10−5 M), but all cases more active. Moreover, inhibited FMLP-induced FCS-coated plastic wells, thus showing direct effect PMNs, while had little effect. Confocal microscopy showed that fluorescent entered HUVEC after 10 min incubation. Chol-but acted either activated PMN HUVEC. 3 Chol-but O2−· production myeloperoxidase release evoked FMLP, dose-dependent, not time-dependent, manner active than butyrate. 4 In conclusion, tests butyrate. Thus, might be valid alternative the anti-inflammatory therapy ulcerative colitis, avoiding complications related administration butyrate. British Journal Pharmacology (2006) 148, 648–656. doi:10.1038/sj.bjp.0706761