In vitro effects of cholesteryl butyrate solid lipid nanospheres as a butyric acid pro-drug on melanoma cells: evaluation of antiproliferative activity and apoptosis induction.

摘要: Literature data show that butyric acid derivatives bear a dose-dependent differentiative anti-proliferative activity on cancer cell lines and apoptosis induction may play major role. Although it was recently shown solid lipid nanospheres (SLNs) are suitable tool for several in vivo drug administration routes, there is little available information melanoma lines. This study aimed at evaluating the apoptotic vitro effects of cholesteryl butyrate (chol-but) SLNs cells. Increasing concentrations chol-but were used to test two Both treated with Na-butyrate (Na-but) viability. Those tested more effective than Na-butirate (3 72 h). The evaluated between 3 72 h by annexin-V (ANX-V)/propidium iodide (PI) staining antiproliferative effect PI staining. Apoptosis anti-proliferative-regulatory proteins as bcl-2, Fas/APO1 (CD95) PCNA (PC10) also investigated. Flow cytometric analyses evidenced G0/1-S transition block `sub-G0/1' peak from 0.5 1.0 mM acid. In ANX-V/PI flow staining, dose- time-dependent increase percentage (ANX-V+) coupled down-regulation PC10 bcl-2 parallel up-regulation found both started after 24 h treatment. Results exerts dose/time-dependent dose but not