Production de polyhydroxyalkanoate (PHA) en utilisant les eaux usées comme source de carbone et les boues activées comme source de micro-organismes.

摘要: L'utilisation generalisee des polyhydroxyalcanoates (PHA) est actuellement limitee par les couts de production eleves ces bioplastiques qui, pourtant ont beaucoup d’avantages par rapport aux matieres plastiques conventionnelles. Des efforts importants ont ete faits pour reduire les production. Ce document passe en revue une nouvelle approche impliquant l'emploi boues activees comme sources microorganismes et / ou dechets substrats la PHAs. D'un point de vue economique, le cout du substrat (principalement source carbone) contribue de facon tres significative a l'ensemble prix d’obtention Il estime a environ 40% total Durant derniere decennie, une grande variete carbone peu onereux (par exemple, renouvelables de carbone), telles que eaux usees, municipaux, agricoles et industriels, des residus sous-produits, carbones (dechets alimentaires produits) ont testes PHAs cultures pures raison leur faible prix potentiel. De cette facon, industriels agricoles sont convertis ayant valeur elevee durable. Dans ce sens, type de recyclage peut contribuer resoudre probleme environnemental leur gestion simultanement aider Le processus de production base sur microbiennes mixtes l'etude comme technologie realiste diminuer , puisque sterilisation n’est pas exigee bacteries peuvent bien s'adapter complexite substrats presents dans dechets; contenu pouvant atteindre environ 70% poids sec cellules. pourrait donc etre competitif avec pures synthetisant plein developpement. Ces approches l'avantage d'economiser volume des dechets extraction stocker Abstract There has been considerable interest in the development of biodegradable plastics since last few decades due to problem and harmful effects conventional on the environment. Poly(3-hydroxyalkanoate) (PHA), one biocompatible thermoplastics, attracted industrial attention. Current methods for PHA production at scale are mostly pure requiring carbon sources and complex costly processing procedures. Therefore, attempts have made to develop more cost-effective processes. PHA processes based mixed microbial (i.e. activated sludge) being investigated as possible technology to reduce costs, since no sterilization is required bacteria can adapt well to the substrates that may be present waste material. This would allow the production from renewable streams, with reduced costs on materials processing. In most studies by activated sludge, synthetic wastewaters were used cultivate producing sludge. this study, the new approach involves use sludge fullscale wastewater treatment plant microorganisms wastewater substrate for investigated. Four types different full-scale plants (municipal, pulp paper industry, starch manufacturing cheese manufacturing wastewaters) showed good potential to produce shake flask experiments. Pulp wastewater sludge was found accumulate maximum concentration. Activated industry plants was used produce 25oC, pH 7 initial sludge suspended solids (SS) concentration (5, 10, 15 20 g/l) computer controlled fermentors (15 L). Acetate sole varying (5 to 20 g/l). Impact feeding pattern polyhydroxyalkanoates was investigated. Batch, fed-batch continuous feed strategies conducted. The maximum content obtained 60.9% (of solid-SS) the sludge SS g/l, acetic acid under C/N ratio 144. While beginning experiment, acetic acid 144, reached 54.9%. Six accumulating bacterial strains isolated and their capabilities had evaluated. The amounts produced significantly varied among six strains. 35.45% was obtained strain PHA-P5 even uncontrolled conditions dissolved oxygen concentration. These identified their 16S rDNA sequences as Comamonas sp. Twelve municipal and the 27.5% PHA-M3. sludge microorganisms also 16S rDNA PHAM3 with Citrobacter sp.. The results study promising activated sludge production, value added product the waste management.