Expression profiling reveals off-target gene regulation by RNAi
作者： Aimee L Jackson , Steven R Bartz , Janell Schelter , Sumire V Kobayashi , Julja Burchard
DOI: 10.1038/NBT831
关键词:
摘要: RNA interference is thought to require near-identity between the small interfering RNA (siRNA) and its cognate mRNA. Here, we used gene expression profiling to characterize the specificity of gene silencing by siRNAs in cultured human cells. Transcript profiles revealed siRNA-specific rather than target-specific signatures, including direct silencing of nontargeted genes containing as few as eleven contiguous nucleotides of identity to the siRNA. These results demonstrate that siRNAs may cross-react with targets of limited …
nature.com
harvard.edu 
sci-hub.se 参考文章(8)
Timothy R. Hughes, Mao Mao, Allan R. Jones, Julja Burchard, Matthew J. Marton, Karen W. Shannon, Steven M. Lefkowitz, Michael Ziman, Janell M. Schelter, Michael R. Meyer, Sumire Kobayashi, Colleen Davis, Hongyue Dai, Yudong D. He, Sergey B. Stephaniants, Guy Cavet, Wynn L. Walker, Anne West, Ernest Coffey, Daniel D. Shoemaker, Roland Stoughton, Alan P. Blanchard, Stephen H. Friend, Peter S. Linsley, Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer. Nature Biotechnology. ,vol. 19, pp. 342- 347 ,(2001) , 10.1038/86730
J. Couzin, Small RNAs Make Big Splash Science. ,vol. 298, pp. 2296- 2297 ,(2002) , 10.1126/SCIENCE.298.5602.2296
Sayda M Elbashir, Javier Martinez, Agnieszka Patkaniowska, Winfried Lendeckel, Thomas Tuschl, Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate The EMBO Journal. ,vol. 20, pp. 6877- 6888 ,(2001) , 10.1093/EMBOJ/20.23.6877
L. A. Martinez, I. Naguibneva, H. Lehrmann, A. Vervisch, T. Tchenio, G. Lozano, A. Harel-Bellan, Synthetic small inhibiting RNAs: Efficient tools to inactivate oncogenic mutations and restore p53 pathways Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 14849- 14854 ,(2002) , 10.1073/PNAS.222406899
Gyorgy Hutvagner, Phillip D Zamore, A microRNA in a Multiple-Turnover RNAi Enzyme Complex Science. ,vol. 297, pp. 2056- 2060 ,(2002) , 10.1126/SCIENCE.1073827
Eric C. Lai, Micro RNAs are complementary to 3' UTR sequence motifs that mediate negative post-transcriptional regulation. Nature Genetics. ,vol. 30, pp. 363- 364 ,(2002) , 10.1038/NG865
Sayda M Elbashir, Jens Harborth, Klaus Weber, Thomas Tuschl, Analysis of gene function in somatic mammalian cells using small interfering RNAs Methods. ,vol. 26, pp. 199- 213 ,(2002) , 10.1016/S1046-2023(02)00023-3
T. Tuschl, P. D. Zamore, R. Lehmann, D. P. Bartel, P. A. Sharp, Targeted mRNA degradation by double-stranded RNA in vitro Genes & Development. ,vol. 13, pp. 3191- 3197 ,(1999) , 10.1101/GAD.13.24.3191