Adipose-derived stromal cell secretome reduces TNFα-induced hypertrophy and catabolic markers in primary human articular chondrocytes

摘要: Abstract Recent clinical trials show the efficacy of Adipose-derived Stromal Cells (ASCs) in contrasting osteoarthritis scenario. Since it is quite accepted that ASCs act predominantly through a paracrine mechanism, their secretome may represent valid therapeutic substitute. The aim this study was to investigate effects ASC conditioned medium (ASC-CM) on TNFα-stimulated human primary articular chondrocytes (CHs). CHs were treated with 10 ng/ml TNFα and/or ASC-CM (1:5 recipient:donor cell ratio). treatment blunted TNFα-induced hypertrophy, reducing levels Osteocalcin (−37%), Collagen X (−18%) and MMP-13 activity (−61%). In addition, decreased MMP-3 by 59%. We showed reduction MMP correlates abundance TIMPs (Tissue Inhibitors MMPs) (with TIMP-1 exceeding 200 ng/ml TIMP-2/3 ng/ml range) rather than direct down-modulation expression release these proteases. contains high other cartilage protecting factors, i.e. OPG DKK-1. comprises cartilage-protecting factors exerts anti-hypertrophic anti-catabolic vitro. Our results support future use cell-derived but cell-free product as approach management osteoarthritis.