Immortalized human hepatocytes as a tool for the study of hepatocytic (de-)differentiation
作者： IJ Schippers , H Moshage , H Roelofsen , M Müller , HSA Heymans
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摘要: Primary human hepatocytes were immortalized by stable transfection with a recombinant plasmid containing the early region of simian virus (SV) 40. The cells cultured in serum-free, hormonally defined medium during immortalization procedure. Foci dividing seen after 3 months. Albumin- and fibrinogen-secreting selected cloned limiting dilution to obtain homologous cell populations. established IHH (immortalized hepatocyte) lines evaluated for their usefulness studying regulation growth certain differentiated hepatocyte functions.
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