Enhancement of neutrophil adherence to isolated rat liver sinusoidal endothelial cells by supernatants of lipopolysaccharide-activated monocytes

摘要: Summary Supernatants of endotoxin-activated monocytes have been shown to stimulate human neutrophil adherence rat liver sinusoidal endothelial cells 3–4-fold. Evidence will be presented that tumor necrosis factor (TNF) is responsible for this phenomenon: (a) in high-performance gel filtration supernatants lipopolysaccharide-activated monocytes, adhesion-inducing activity coeluted with TNF measured the L929 cell-lysing assay at 25–45 kDa; (b) anti-TNF antibody treatment activated macrophages abolished their activity; (c) recombinant alpha stimulated adhesion a dose-dependent manner. In addition, polymyxine B sulfate, which was capable neutralizaing direct effects lipopolysaccharide on adhesion, could abolish neither neutrophil-adhesion-inducing nor effect itself. The due both activation neutrophils and an influence endothelium itself by TNF: pretreatment followed thorough washing resulted increased attachment. Protein synthesis not required. However, incubation abrogated adhesion. This suggests bound cell surfaces It concluded increasing granulocyte sticking lining sinusoids may play significant role endotoxin-induced inflammation as it found septic state.