Overexpression and purification of the particulate methane monooxygenase from Methylococcus capsulatus (Bath).
作者： Sunney I. Chan , H.-Hoa T. Nguyen , Kelvin H.-C. Chen , Steve S.-F. Yu
DOI: 10.1016/B978-0-12-386905-0.00012-7
关键词:
摘要: The particulate methane monooxygenase (pMMO) is a multi-copper enzyme that mediates the facile conversion of to methanol in methanotrophic bacteria. As membrane-bound multi-subunit metalloprotein, highly active protein has been difficult isolate and purify homogeneity for biochemical biophysical studies. In this chapter, we describe method overexpress pMMO with good specific activity high yields intracytoplasmic membranes host organism, together two protocols from pMMO-enriched without loss copper cofactors enzymatic activity.
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