Quantitative proteomic analysis of metabolic regulation by copper ions in Methylococcus capsulatus (Bath)
作者： Wei-Chun Kao , Yet-Ran Chen , Eugene C. Yi , Hookeun Lee , Qiang Tian
关键词:
摘要: Copper ions switch the oxidation of methane by soluble monooxygenase to particulate in Methylococcus capsulatus (Bath). Toward understanding change cellular metabolism related this transcriptional and metabolic switch, we have undertaken genomic sequencing quantitative comparative analysis proteome M. (Bath) grown under different copper-to-biomass ratios cleavable isotope-coded affinity tag technology. Of 682 proteins identified, expressions 60 were stimulated at least 2-fold copper ions; 68 down-regulated or more. The overexpressed included carbohydrate enzymes, while suppressed mainly responsible for signaling processes, indicating a role expression genes associated with organism downstream oxidation. study has also provided complete map C1 pathways methanotroph clarified interrelationships between them.
nhri.org.tw参考文章(35)
C. Anthony, The Biochemistry of Methylotrophs ,(1982)
M Takagi, H Takada, T Imanaka, Nucleotide sequence and cloning in Bacillus subtilis of the Bacillus stearothermophilus pleiotropic regulatory gene degT. Journal of Bacteriology. ,vol. 172, pp. 411- 418 ,(1990) , 10.1128/JB.172.1.411-418.1990
J R Quayle, T Ferenci, Evolutionary aspects of autotrophy. Microbiological Research. ,vol. 42, pp. 251- 273 ,(1978) , 10.1128/MR.42.2.251-273.1978
Sunney I. Chan, Kelvin H.-C. Chen, Steve S.-F. Yu, Chang-Li Chen, Simon S.-J. Kuo, Toward delineating the structure and function of the particulate methane monooxygenase from methanotrophic bacteria. Biochemistry. ,vol. 43, pp. 4421- 4430 ,(2004) , 10.1021/BI0497603
Peter Rice, Ian Longden, Alan Bleasby, EMBOSS: The European Molecular Biology Open Software Suite Trends in Genetics. ,vol. 16, pp. 276- 277 ,(2000) , 10.1016/S0168-9525(00)02024-2
R. A. Kerr, PLANETARY SCIENCE: Life or Volcanic Belching on Mars? Science. ,vol. 303, pp. 1953- 1953 ,(2004) , 10.1126/SCIENCE.303.5666.1953
Anne Fjellbirkeland, Per Kruger, Vahid Bemanian, Bente Høgh, Colin Murrell, Harald Jensen, The C-terminal part of the surface-associated protein MopE of the methanotroph Methylococcus capsulatus (Bath) is secreted into the growth medium. Archives of Microbiology. ,vol. 176, pp. 197- 203 ,(2001) , 10.1007/S002030100307
Junjie Xiong, Donald M. Kurtz,, Jingyuan Ai, Joann Sanders-Loehr, A hemerythrin-like domain in a bacterial chemotaxis protein. Biochemistry. ,vol. 39, pp. 5117- 5125 ,(2000) , 10.1021/BI992796O
Róbert Csáki, Levente Bodrossy, József Klem, J. Colin Murrell, Kornél L. Kovács, Genes involved in the copper-dependent regulation of soluble methane monooxygenase of Methylococcus capsulatus (Bath): cloning, sequencing and mutational analysis Microbiology. ,vol. 149, pp. 1785- 1795 ,(2003) , 10.1099/MIC.0.26061-0
Alexey I. Nesvizhskii, Andrew Keller, Eugene Kolker, Ruedi Aebersold, A statistical model for identifying proteins by tandem mass spectrometry. Analytical Chemistry. ,vol. 75, pp. 4646- 4658 ,(2003) , 10.1021/AC0341261