Serum IgG to heat shock proteins and Porphyromonas gingivalis antigens in diabetic patients with periodontitis.

摘要: Background:Past studies have reported a correlation between the presence and severity of periodontitis serum antibody titers to species-specific antigens Porphyromonas gingivalis or cross-reactive antigens, such as lipopolysaccharide (LPS) heat shock proteins (HSP), shared P. other bacteria. Our recent study periodontal treatment outcome in insulin-dependent (type 1) diabetes mellitus patients with severe (IDDMI/periodontitis) resulted two key findings: 1. glutamic acid decarboxylase autoantibody (GAD65 Ab) levels were significantly associated pocket depth change (PDC) 2. IgG cells positively GAD65 Ab level seropositive +) patients. We therefore hypothesized that profiles titers, gingivalis-specific may be useful assessing risk for refractory Aim:To determine whether PDC resulting from non-surgical can predicted using baseline gingivalisspecific human HSP, GAD65. Methods:PDC was assessed months after 7 + 11 AbIDDM/periodontitis Pretreatment GAD65, recombinant (HSP90, HSP70, HSP60), various measured radioligand precipitation enzyme-linked immunosorbent (ELISA) assays compared same measurements 154 recent-onset IDDM 46 non-diabetic controls. Results:Median (ELISA units) HSP90 HSP70 higher than controls (p°= 0.002) Ab- (p = 0.034) IDDM/periodontitis patients, respectively. Multivariate regression analysis indicated significant partial log-transformed (r − 0.62, p 0.008), 0.009), 0.60, 0.01) LPS 0.5 1, 0.04). Furthermore, hierarchical clustering HSP90, sorted into distinct clusters different median (1.45 min, n 10 vs. 0.65 8; 0.016, Mann–Whitney). Conclusion:Pretreatment predicting which will poor response therapy. (Less)