Fluorescence suppression using micro-scale spatially offset Raman spectroscopy

摘要: We present a new concept of fluorescence suppression in Raman microscopy based on micro-spatially offset spectroscopy which is applicable to thin stratified turbid (diffusely scattering) matrices permitting the retrieval signals sublayers below intensely fluorescing over-layers. The method demonstrated yield good quality spectra with dramatically suppressed backgrounds enabling sublayer even situations where conventional are fully overwhelmed by intense fluorescence. performance was studied theoretically using Monte Carlo simulations indicating potential up an order or two magnitude overlayer relative signals. technique applicability conceptually layered samples involving paints, polymers and stones yielding factors between 12 above 430. has applications number analytical areas including cultural heritage, archaeology, polymers, food, pharmaceutical, biological, biomedical, forensics catalytic sciences control manufacture.