Pros and cons of using genomic SSRs and EST-SSRs for resolving phylogeny of the genus Gossypium

摘要: The genus Gossypium is comprised of 50 diverse cotton species representing eight different genomes (A through G and K), however, phylogenetic relationship using various DNA marker types such as RAPD SSRs was determined on limited number species. In this report, we have demonstrated the application genomic (gSSRs) EST-SSRs, after combining both data sets, for resolving phylogenies 36 including seven races. Out 100 primer pairs surveyed (50 gSSRs EST-SSRs), 75 produced scorable amplification products in all these, 73 were found to be polymorphic amplified 135 alleles ranging from 1 5 per SSR (average 2.87 marker). higher (72) compared EST-SSRs (63). total 22 highly informative with PIC values ≥0.5 identified. Genomic containing di-while tri-nucleotide repeats exhibited high polymorphism other nucleotide gSSRs/EST-SSRs. Number tandem positively correlated. Neither type chromosome nor location showed association polymorphism. herbaceum var. africanum (Watt) Hutch. ex Ghose robinsonii F. Muell. most genetically diverse, while among races hirsutum L. “yucatanense” G. “punctatum” diverse. Of three clustering analysis based combined revealed parallel results reported earlier studies. This study further confirmed that darwinii Watt has close barbadense Moreover, raimondii Ulbr. herbaceum/Gossypium arboreum are living relatives ancestor allotetraploid Our studies suggest plant could a better choice. information can instrumental transferring novel or loci wild into cultivated which would set stage cultivating cultivars—a way achieve sustainable production changing climate.