Elastase-mediated Release of Heparan Sulfate Proteoglycans from Pulmonary Fibroblast Cultures A MECHANISM FOR BASIC FIBROBLAST GROWTH FACTOR (bFGF) RELEASE AND ATTENUATION OF bFGF BINDING FOLLOWING ELASTASE-INDUCED INJURY
作者： Jo Ann Buczek-Thomas , Matthew A. Nugent
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摘要: We have investigated elastase-mediated alterations in the expression of basic fibroblast growth factor (bFGF) receptors and proteoglycan co-receptors characterized subsequent effects on bFGF receptor binding profiles. For these studies, pulmonary cultures were treated with porcine pancreatic elastase, changes profiles assessed. Quantitation [(35)S]sulfate-labeled total glycosaminoglycan release from matrices indicated that elastase treatment released sulfated cell surface a time- dose-dependent fashion correlated strongly release. Ligand studies decreased (125)I-bFGF to affected both heparan sulfate (HSPG) sites. Western blot analyses did not significant amounts protein. These findings indicate HSPG reduces effective affinity for its receptor. Collectively, suggest are important mediators response bFGF, HSPG, other elastase-released entities play an role lung chronic injury.
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