Separation of Parafollicular Cells from Thyroid Follicular Cells by Affinity Chromatography Using Thyroglobulin-Coupled Sepharose

摘要: Publisher Summary This chapter discusses a separation technique for of parafollicular cells from thyroid follicular by affinity chromatography using thyroglobulin-coupled sepharose. The parenchyma is comprised two cell types: and cells. These are enmeshed in highly vascularized connective tissue stroma that contains, addition to the usual cellular fibrous components, large number mast Parafollicular produce, store, secrete calcitonin. originate neural crest and, manner reminiscent enteric neurons, associate with endodermally derived parenchymal mesenchymal stroma. do not differ very much size. They both approximately 10 μm diameter at their widest point. In an experiment described chapter, it was found internalized silica instead resulting denser cell; toxic caused breakage lysosomes subsequent release hydrolytic enzymes into cytosol. An column composed sepharose successful retarding movement TSH-stimulated Consequently, ratio considerably increased certain fractions effluent, as were concentration 5-HT activity SBP.