Intracellular distribution of amines and calcitonin in the sheep thyroid gland.

摘要: The distribution of amines (dopamine, histamine, 5-hydroxytryptamine, and norepinephrine) calcitonin in the sheep thyroid gland was studied by quantitative analyses these substances subcellular fractions examined with electron microscope. were prepared centrifugation continuous sucrose gradients. Histamine mainly found supernatant fraction dopamine gradient pellet together mast cell granules. This indicates a release but not dopamine, from granules, which contain both amines. Norepinephrine recovered adjacent fraction. 5-Hydroxytryptamine concentrated one contained high concentration dense, argentaffin indistinguishable those sections parafollicular cells. These observations show that 5-hydroxytryptamine polypeptide are stored almost exclusively cytoplasmic granules