Genome‐wide methylation analysis in vestibular schwannomas shows putative mechanisms of gene expression modulation and global hypomethylation at the HOX gene cluster

摘要: Schwannomas are tumors that develop from Schwann cells in the peripheral nerves and commonly arise vestibular nerve. Vestibular schwannomas can present unilaterally sporadically or bilaterally when tumor is associated with neurofibromatosis Type 2 (NF2) syndrome. The molecular hallmark of disease biallelic inactivation NF2 gene. epigenetic signature remains poorly understood mostly limited to DNA methylation gene, whose altered expression due factors this controversial. In study, we tested genomewide pattern shed light on alteration these particular tumors. methodology used includes Infinium Human Methylation 450K BeadChip microarrays a series 36 schwannomas, 4 nonvestibular 5 healthy nerves. Our results show trend toward hypomethylation schwannomas. Furthermore, homeobox (HOX) genes, located at four clusters genome, displayed several CpG sites but not Several microRNA (miRNA) protein-coding genes were also found be hypomethylated promoter regions confirmed as upregulated by analysis; including miRNA-21, Met Proto-Oncogene (MET), PMEPA1. We detected patterns might involved alternative transcripts such NRXN1 MBP, which would increase complexity patterns. Overall, our specific signatures coding miRNAs could potentially therapeutic targets.