Effects of Globin mRNA Reduction Methods on Gene Expression Profiles from Whole Blood

摘要: Excessive globin mRNA in whole blood RNA decreases transcript detection sensitivity and increases signal variation on microarrays. Hence, methods based peptide nucleic acid inhibitory oligos biotinylated DNA capture have been developed to reduce mRNA. However, there is limited information about the effects of these two gene expression profiles. Thus, we systematically compared facility reduction profile measurements from Jurkat cell line with or without spiked human isolated using PAXgene collection tubes. We showed that were efficient at increasing loss specificity, but neither method could recover a equivalent an identical sample excesses. The oligo had slightly better for RNA, lowered more similar profiles controls than method. required larger amounts initial high-quality yield sufficient cRNA amounts, its procedures complex time consuming These results inform selection suitable multicenter surveillance