Gene Expression Patterns of Dengue Virus-Infected Children from Nicaragua Reveal a Distinct Signature of Increased Metabolism

摘要: Author(s): Loke, P'ng; Hammond, Samantha N; Leung, Jacqueline M; Kim, Charles C; Batra, Sajeev; Rocha, Crisanta; Balmaseda, Angel; Harris, Eva | Abstract: BackgroundInfection with dengue viruses (DENV) leads to a spectrum of disease outcomes. The pathophysiology severe versus non-severe manifestations DENV infection may be driven by host responses, which could reflected in the transcriptional profiles peripheral blood immune cells.Methodology/principal findingsWe conducted genome-wide microarray analysis whole RNA from 34 DENV-infected children Nicaragua collected on days 3-6 illness, different manifestations. Gene expression identified genes that are differentially regulated between clinical subgroups. most striking differences were observed patients and without shock, especially mitochondrial ribosomal proteins associated protein biosynthesis. In hemorrhagic fever patients, one subset expressed encode neutrophil-derived anti-microbial peptides innate immunity. By performing meta-analysis our dataset conjunction previously published datasets, we confirmed vivo is large changes nucleic acid metabolism. Additionally, whereas vitro an increased interferon signature, this was not consistently patient samples, suggesting response relatively transient no longer illness.Conclusions/significanceThese data highlight important severity during as well identify some commonalities. Compilation larger datasets future across multiple studies, have initiated report, lead better prediction manifestation via systems biology approach.