Dengue Virus Infection Induces Expansion of a CD14+CD16+ Monocyte Population that Stimulates Plasmablast Differentiation

摘要: Summary Dengue virus (DENV) infection induces the expansion of plasmablasts, which produce antibodies that can neutralize DENV but also enhance disease upon secondary with another serotype. To understand how these immune responses are generated, we used a systems biological approach to analyze dengue in humans. Transcriptomic analysis whole blood revealed genes encoding proinflammatory mediators and type I interferon-related proteins were associated high levels during initial symptomatic disease. Additionally, CD14 + CD16 monocytes increased blood. Similarly, nonhuman primate model, boosted monocyte numbers lymph nodes. Upon in vitro, upregulated mediated differentiation resting B cells plasmablasts as well immunoglobulin G (IgG) IgM secretion. These findings provide detailed picture innate highlight a role for promoting plasmablast anti-DENV antibody responses.