The influence of calcium, magnesium and cyclic adenosine 3',5'-monophosphate on the mixed lymphocyte reaction.

摘要: EDTA inhibits lymphocyte transformation in the mixed culture reaction as it does PHA containing cultures. The effect is most likely due to binding of Ca ++ and Mg required for initiation completion response since not occur cultures deficient these ions. Exogenous cyclic adenosine 3′,5′-monophosphate (cAMP) can potentiate or inhibit depending on concentration duration exposure. possible interaction divalent cations cAMP discussed.