Rab11-FIP3 is a cell cycle-regulated phosphoprotein

摘要: Rab11 and its effector molecule, Rab11-FIP3 (FIP3), associate with recycling endosomes traffic into the furrow midbody of cells during cytokinesis. FIP3 also controls endosome distribution interphase. Here, we examine whether phosphorylation is involved in these activities. We identify four sites vivo, S-102, S-280, S-347 S-450 S-102 as a target for Cdk1-cyclin B vitro. Of these, show that phosphorylated metaphase dephosphorylated enter telophase. Over-expression FIP3-S102D increased frequency binucleate consistent role this phospho-acceptor site Mutation or other previously identified (S-488, S-538, S-647 S-648) was without effect on cell formation did not modulate cycle. In an attempt to functional phosphorylation, report change from cytosolic membrane-associated observed progression anaphase telophase accompanied by concomitant dephosphorylation FIP3. However, here control distribution. Our data thus cycle regulated phosphoprotein suggest accompanies translocation cytosol membranes S102 telophase; mutation exerts modest Finally, de/phosphorylation (S-102, S-450) required spatial function.