Additively manufactured 3D porous Ti-6Al-4V constructs mimic trabecular bone structure and regulate osteoblast proliferation, differentiation and local factor production in a porosity and surface roughness dependent manner

摘要: Additive manufacturing by laser sintering is able to produce high resolution metal constructs for orthopedic and dental implants. In this study, we used a human trabecular bone template design manufacture Ti-6Al-4V with varying porosity via sintering. Characterization of revealed interconnected porosities ranging from 15–70% compressive moduli 2579–3693 MPa. These macro were further surface-treated create desirable multi-scale micro-/nano-roughness, which has been shown enhance the osseointegration process. Osteoblasts (MG63 cells) exhibited viability when grown on constructs. Proliferation (DNA) alkaline phosphatase specific activity, an early differentiation marker, decreased as increased, while osteocalcin, late well osteoprotegerin, vascular endothelial growth factor morphogenetic proteins 2 4 increased increasing porosity. Three-dimensional (3D) highest surface modification supported greatest osteoblast local production. results indicate that additively manufactured 3D porous mimicking produced additional treatment can be customized response. Increased factors maturation suggest enhanced performance these surfaces in vivo.