THE ROLE OF AMINO GROUPS IN THE BIOLOGICAL AND ANTIGENIC ACTIVITIES OF CLOSTRIDIUM PERFRINGENS TYPE A ENTEROTOXIN
作者： JOHN R. WHITAKER , PER EINAR GRANUM
DOI: 10.1111/J.1745-4514.1980.TB00781.X
关键词:
摘要: Clostridium perfingens type A enterotoxin has 8 amino groups which are readily reactive with 2, 4, 6-trinitrobenzene sulfonic acid (TNBS) in 20 mM phosphate buffer, pH 6.8, and 10 react TNBS only after denaturation 6.5 M guanidine · HCl ≤ 25°C. The one sulfhydryl group of is not 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) the native molecule (phosphate 6.8), but presence 0.2% sodium dodecyl sulfate at 25°C. i
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