Influence of subtilisin on the adhesion of a marine bacterium which produces mainly proteins as extracellular polymers.
作者： C. Leroy , C. Delbarre , F. Ghillebaert , C. Compere , D. Combes
DOI: 10.1111/J.1365-2672.2008.03837.X
关键词:
摘要: Aims: The nature of exopolymers involved in the adhesion a marine biofilm-forming bacterium Pseudoalteromonas sp. D41 was investigated to evaluate and understand antifouling potential subtilisin. Methods Results: produced by fermentation were analysed FTIR SDS-PAGE showing presence polysaccharides, glycoproteins proteins. A high content proteins detected both soluble capsular fractions. microscopic observations fluorescamine calcofluor stained adhered indicated mainly during adhesion. Subtilisin, broad spectrum protease, tested natural sea water polystyrene microplates showed that activity higher prevention bacterial than detachment cells. Conclusions: Overall, these results demonstrate involvement confirm Significance Impact Study: This study emphasizes major role instead thus extending our knowledge regarding extracellular polymers Furthermore, subtilisin evaluated very first stages fouling, adhesion, could lead less toxic compounds organometallic paint.
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