Plant regeneration from Eucalyptus bosistoana callus culture

摘要: Eucalyptus bosistoana is an important durable hardwood species selected by New Zealand Dryland Forest Initiative (NZDFI) for their adaptability to diverse environments and good quality wood. There no report on in vitro methods callus induction plantlet micropropagation of this species, although these could assist further genetic improvement plants. In study, different combinations α-naphthalene acetic acid (NAA) benzyl adenine (BA) at concentrations ranging from 0.5 2 mg L−1 were investigated induction, shoot regeneration, elongation root formation. High frequency was obtained when cotyledon explants cultured MS (Murashige Skoog medium 1962) with 1.0 mg L−1 NAA 1.5 mg L−1 BA. For regeneration callus, 0.5 mg L−1 BA effective medium. Shoot observed supplemented NAA, GA3. adventitious formation micro-cuttings E. half-strength NAA. Finally, pre-acclimatization the micropropagated plantlets led 100% survival under glasshouse conditions.