Micropropagation of Eucalyptus camaldulensis for the production of rejuvenated stock plants for microcuttings propagation and genetic fidelity assessment

摘要: Adventitious rooting, a key step in clonal propagation is affected by tree maturation. Micropropagation followed microcuttings has the potential to rejuvenate clones thereby enhancing rooting and minimize intra variation. In this study, 33 superior performing Eucalyptus camaldulensis were propagated of stem cuttings (SCs) micropropagation. Micropropagated plantlets used as stock plants for microcutting propagation. Rooting SCs micropropagation was carried out with coppice shoot axillary buds respectively, obtained from approximately fourteen year old trees that had undergone one vegetative cycle. The adventitious recorded significantly higher (24.8–100 %) (43–95 %) than method (9.3–75.5 %). Studies on ontogeny showed emergence root primordium phloem region initials noticed within 5–9 days after auxin treatment. Further, molecular marker analysis genetic uniformity except two ramets, detected using inter simple sequence repeats (ISSR) markers suitable corrective measures taken avoid entry of such mass multiplication. This study demonstrates importance integration production rejuvenation Although current induction treatments favored basic mechanisms involved need be explored hassle free industrial process, consequently cost effective