Isolation and regeneration of protoplasts from higher plants.

摘要: The aseptic culture of plant cells has emerged in recent years as a powerful technique not only for the study cell differentiation, but also improvement and agriculture. During past few years, potential vastly improved due to fast emerging technology isolation, cultivation, fusion, protoplasts. term “protoplast” this respect refers spherical plasmolyzed contents enclosed plasmalemma set free covering wall by suitable experimental method. naked so obtained constitute an ideal “free cell” developmental system because protoplasts are separate entities capable reforming walls regenerating whole plants. This fact is considerable advantage clonal, large scale, propagation desired plants become much more efficient, being discrete, — especially from haploid or lines can be handled like microbes well suited mutagenic treatments somatic genetics.