Cell behaviour on micropatterned substrata: limits of extracellular matrix geometry for spreading and adhesion

摘要: Cell adhesion, spreading and migration require the dynamic formation dispersal of contacts with extracellular matrix (ECM). In vivo, number, availability distribution ECM binding sites dictate shape a cell determine its mobility. To analyse geometrical limits required for attachment spreading, we used microcontact printing to produce regular patterns protein dots defined size separated by nonadhesive regions. Cells cultured on these substrata adhere spread regions as small 0.1 microm2, when spacing between is less than 5 microm. Spacing 5-25 microm induces adapt pattern. The ability migrate > or =1 microm2 ceases dot separation =30 extent directly correlated total substratum coverage ECM-proteins, but irrespective An optimal reached at surface coating above 15%. Knowledge essential an understanding adhesion migration, design artificial surfaces that optimally interact cells in living tissue.