Clues About Chromoblastomycotic and Other Dematiaceous Fungal Pathogens Based on Wangiella as a Model

摘要: Wangiella dermatitidis is an excellent model for discovering biologically and medically relevant information about other dematiaceous agents of mycosis. The major attribute this phaeohyphomycotic fungus, which allows it to serve as a useful model, its vegetative polymorphism. This polymorphism expressed both in vivo vitro programs number developmental choices leading polarized growth by budding hyphal apical extension or type non-polarized, isotropic that produces spherically enlarged cells multicellular forms. latter phenotypes are virtually identical the sclerotic bodies chromoblastomycotic fungi. Study phenotypic switching between forms has been possible because can be induced form from yeasts hyphae manipulation culture medium pH, incubation certain cell-divisioncycle mutants at elevated temperatures. It evident particular dimorphism involves considerable rearrangement cell-wall synthetic deposition patterns, results inhibition extension, without growth, nuclear division cytokinesis. also becoming Ca2+ may have regulatory role switching, new showed acidic pH low, but critical, concentration was crucial multicellular-body development, higher concentrations allowed maintenance Ca2+/proton exchange mechanism might involved. Support important provided with EGTA. At high near neutral chelator caused stage-specific, cell-cycle arrest yeast cells, lower increasing numbers convert Parallel studies fungi maintained very withholding low EGTA more isotropism formation bodies. These preliminary further validate concept W. continues pathogens humans.