Relationship between the outcomes of assisted reproductive techniques and sperm DNA fragmentation as measured by the sperm chromatin structure assay

摘要: Abstract Objective To investigate how moderate and/or high levels of DNA fragmentation (DFI), as measured by the sperm chromatin structure assay (SCSA), affect either IVF or with intracytoplasmic injection (ICSI) fertilization, cleavage, blastulation, implantation, and pregnancy. Design: Retrospective clinical study. Setting: Academic human reproduction laboratory. Patient(s): Eighty-nine couples undergoing conventional fertilization ICSI. Intervention(s): Sperm testing (SCSA) semen aliquot taken from ejaculate used for assisted reproductive technology (ART). Main outcome measure(s): Related DFI to parameters cycle-specific outcomes after ART. Result(s): No patients achieved pregnancy if SCSA values exceeded (27%, P r 2 ) between 0.29. Conclusion(s): identified thresholds negative ART not using parameters. This is first study analyzing value [1] include a large number (n = 89), [2] perform analysis on ART, [3] examine extent (moderate DFI) influenced outcomes.