Codon optimization of the human papillomavirus E7 oncogene induces a CD8+ T cell response to a cryptic epitope not harbored by wild-type E7.

摘要: Codon optimization of nucleotide sequences is a widely used method to achieve high levels transgene expression for basic and clinical research. Until now, immunological side effects have not been described. To trigger T cell responses against human papillomavirus, we incubated cells with dendritic that were pulsed RNA encoding the codon-optimized E7 oncogene. All receptors isolated from responding clones recognized target expressing gene but wild type sequence. Epitope mapping revealed recognition cryptic epitope +3 alternative reading frame E7, which encoded by The introduction stop codon into protected product receptor gene-modified cells. This first experimental study demonstrating can render artificially immunogenic through generation dominant epitope. finding may be great importance field therapy avoid rejection gene-corrected design DNA- RNA-based vaccines, where add strong component vaccine.