Newcastle disease virus-infected intact autologous tumor cell vaccine for adjuvant active specific immunotherapy of resected colorectal carcinoma.

摘要: An active specific immunization (ASI) procedure with two types of autologous tumor cell vaccines (ATVs) is tested for adjuvant immunotherapy resected colorectal carcinoma to provide preliminary information on local immunological skin responses, side effects, and 2-year survival rates. For vaccine preparation, the tumor-derived freshly isolated cryopreserved cells were thawed, purified by Percoll density centrifugation, depleted tumor-infiltrating lymphocytes immunomagnetic beads. After inactivation 200 Gy, this ATV either infected Newcastle disease virus (NDV) or they admixed Bacillus Calmette Guerin (BCG) organisms. Vaccination was performed in arm beginning 6-8 weeks after operation, three times at 2-week intervals. Of 57 patients that received ASI, 48 treated virus-infected (ATV-NDV) 9 BCG-admixed (ATV/BCG). The mean value delayed hypersensitivity reactions from ATV-NDV-treated 18 mm first vaccination 26 29 succeeding ones. Although application ATV-NDV associated only mild ATV/BCG led long-lasting ulcers more serious effects. rate obtained 97.9%, whereas 66.7%. 661 a historical control 73.8%. These data suggest type quality ASI treatment important. findings necessitate corroboration prospective, randomized controlled study.