miR-423 Promotes Breast Cancer Invasion by Activating NF-κB Signaling.

摘要: Objective Breast cancer has become the most common malignancy among women worldwide; therefore, novel diagnostic and prognostic markers therapeutic targets are urgently required. NF-κB signaling plays a pivotal role in enhancing breast malignant phenotypes, especially invasion metastasis, which is main cause of death patients. TNIP2, an important inhibitor pathway, known to involve negative feedback loop cascade regulate tumor aggressiveness various types. However, mRNA level TNIP2 barely altered cancer; thus, mechanism that regulates still needs be elucidated. Methods We analyzed expression prognosis miR-423 TCGA BRCA miRNA cohort clinical specimens. detected invasive capacity through Matrigel-coated Transwell penetration assay, three-dimensional (3D) spheroid assay wound healing assay. Then, we applied luciferase assays, real-time PCR assays Western blotting further study mechanism. Results In this study, analysis specimens demonstrated was upregulated human cancers positively correlated with stage, poor overall survival metastasis classification. Moreover, invasiveness cells enhanced by ectopic inhibited downregulation. Mechanistically, upregulation led activation pathway elevated snail twist, while repression pathway. Furthermore, results indicated target gene miR-423, suppression resulted increased miR-423-silenced cells. Conclusion Our suggest crucial factor enhances cell shed light on as promising marker for metastatic cancer.