Environmental influence on T cell receptor α gene rearrangement and expression in vitro

摘要: Experiments were designed to test whether T cell progenitors are committed particular receptor (TcR) gene rearrangement and expression patterns (prior rearrangement) or such patients molded by the thymic microenvironment in which cells develop. To this end, day 14 fetal thymocytes removed from their normal environment grown organ culture (FTOC) for 5 12 days. RNA was extracted organ-cultured cells, processed cDNA, TcR alpha sequences amplified polymerase chain reaction cloning sequencing. By examination of N-region additions V-gene usage, comparison resultant with those early vs. adult stages differentiation vivo, it demonstrated that FTOC did not maintain rearrangement. The thymocyte most dissimilar normal, ontogeny when interleukin-4 added FTOC. Taken together, results flexibility plays a critical role molding patterns.