TRAIL induces apoptosis and inflammatory gene expression in human endothelial cells.

摘要: Human TRAIL can efficiently kill tumor cells in vitro and human xenografts mice with little effect on normal mouse or tissues. The effects of tissues have not been described. In this study, we report that endothelial (EC), isolated from umbilical veins dermal microvessels, express death domain-containing TRAIL-R1 -R2. Incubation for 15 h causes approximately 30% cultured EC to die, as assessed by propidium iodide uptake. Death is apoptotic, Annexin V staining, 4',6'-diamidino-2-phenylindole DNA fragment ELISA. increased cotreatment cycloheximide but significantly reduced caspase inhibitors transduced dominant-negative Fas-associated domain protein. surviving cells, activates NF-kappaB, induces expression E-selectin, ICAM-1, IL-8, promotes adhesion leukocytes. Injection into skin focal injury accompanied limited neutrophil infiltration. These data suggest an inducer tissue humans, outcome may influence antitumor therapy TRAIL.