Tumoricidal activity of tumor necrosis factor-related apoptosis-inducing ligand in vivo

摘要: To evaluate the utility of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) as a cancer therapeutic, we created leucine zipper (LZ) forms human (hu) and murine (mu) TRAIL to promote stabilize formation trimers. Both were biologically active, inducing apoptosis both target cells in vitro with similar specific activities. In contrast fulminant hepatotoxicity LZ-huCD95L vivo, administration either LZ-huTRAIL or LZ-muTRAIL did not seem toxic normal tissues mice. Finally, repeated treatments actively suppressed growth TRAIL-sensitive mammary adenocarcinoma cell line MDA-231 CB.17 (SCID) mice, histologic examination tumors from SCID mice treated demonstrated clear areas apoptotic within 9-12 hours injection.