Single-Nucleotide Polymorphisms and Other Mismatches Reduce Performance of Quantitative PCR Assays
作者： Steve Lefever , Filip Pattyn , Jan Hellemans , Jo Vandesompele
DOI: 10.1373/CLINCHEM.2013.203653
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摘要: … mixes A, B, D, and E. In addition, a marked reduction in both SD and variation in amplification efficiency were observed for mixes A, D, and E as the mismatch was moved away from the …
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