Quantification of Y chromosome bearing spermatozoa of cattle using in situ hybridization.
作者： Manfred Schwerin , Steffen Blottner , Preben D. Thomsen , Daniela Roschlau , Gudrun Brockmann
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摘要: An easy assay for quantification of Y chromosome-bearing sperm (Y-sperm) is needed, especially to monitor separation techniques. In the present study a tritiated bovine male-specific DNA fragment was tested identification Y-sperm by in situ hybridization. A protocol hybridization developed and used proportion 12 bulls. The usefulness method optimization procedures illustrated through analysis fractions separated Percoll density gradient centrifugation.
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