Incorporation of 4'-C-aminomethyl-2'-O-methylthymidine into DNA by thermophilic DNA polymerases.
作者： Ganesh N. Nawale , Kiran R. Gore , Claudia Höbartner , P. I. Pradeepkumar
DOI: 10.1039/C2CC35222B
关键词:
摘要: The dual modified nucleotide 4′-C-aminomethyl-2′-O-methylthymidine 5′-triphosphate was synthesized and enzymatically incorporated into DNA by the thermophilic polymerases Pfu Therminator III. ribose modification imparted increased exonuclease resistance to compared well-known 2′-O-methyl modification.
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