Shape and Interhelical Spacing of DNA Origami Nanostructures Studied by Small-Angle X-ray Scattering
作者： Stefan Fischer , Caroline Hartl , Kilian Frank , Joachim O. Rädler , Tim Liedl
DOI: 10.1021/ACS.NANOLETT.6B01335
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摘要: Scaffolded DNA origami nanostructures enable the self-assembly of arbitrarily shaped objects with unprecedented accuracy. Yet, varying physiological conditions are prone to induce slight structural changes in nanoscale architecture. Here, we report on high precision measurements overall shape and interhelical distance three prototypic structures solution using synchrotron small-angle X-ray scattering. Sheet-, brick-, cylinder-shaped constructs were assembled factors determined angstrom resolution from fits scattering profiles. With decreasing MgCl2 concentration electrostatic swelling both cross section spacing is observed. The tolerate up 10% expansion before they disintegrate. In contrast, increasing temperature, show no thermal a wide temperature window abruptly melt above 50 °C. Details molecula...
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