Large-scale ordered plastic nanopillars for quantitative live-cell imaging.

摘要: Surfaces exhibiting ordered nanopillars have a wide range of potential biomedical applications based on the altered adhesivity living cells nanopatterned surfaces compared to planar ones. Examples include scaffolding for tissue engineering, designer bandages wound dressing, and antifouling implants. Although numerous experiments performed over last decade confirmed that respond chemistry (biochemical 2D imprint) geometry (topographical 3D relief) their surroundings at nanoscale, fundamental processes by which recognize nanostructures is subject on-going research. In this context, there need ensure nanostructured large-scale coverage are compatible with quantitative optical microscopy (QOM), an important tool studying cells, especially dynamics thereof. While biochemical patterning not expected pose special challenge QOM, topographical may do so. Well-known techniques nanoimprint lithography (NIL, including thermal embossing UV curing) self-assembly colloidal beads or phase separation polymers, all achieve large coverage. NIL relatively resource intensive usually depends conventional like electron-beam initial stamp. Self-assembly, although increasingly refined, has limited flexibility choice motif. Transparent substrates made using these